8VEO
Crystal structure of PRMT5:MEP50 in complex with MTA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 101.680, 138.880, 178.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.790 - 2.030 |
R-factor | 0.2411 |
Rwork | 0.239 |
R-free | 0.27750 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.011 |
RMSD bond angle | 1.041 |
Data reduction software | XDS |
Data scaling software | Aimless |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.790 | 2.080 |
High resolution limit [Å] | 2.030 | 2.030 |
Rmerge | 0.045 | 0.797 |
Rmeas | 0.049 | 0.867 |
Rpim | 0.019 | 0.338 |
Total number of observations | 539109 | 36367 |
Number of reflections | 80230 | 5690 |
<I/σ(I)> | 18 | 2.8 |
Completeness [%] | 98.5 | |
Redundancy | 6.7 | 6.4 |
CC(1/2) | 0.999 | 0.914 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 291 | 10% PEG 4000, 0.2M magnesium chloride, 0.1M sodium citrate (pH 6.0) |