8VDS
DNA Ligase 1 with nick DNA 3'rG:C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE 7B2 |
| Synchrotron site | CHESS |
| Beamline | 7B2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.725, 115.889, 126.206 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.900 - 2.790 |
| R-factor | 0.201 |
| Rwork | 0.196 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.513 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.850 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rpim | 0.033 | 0.310 |
| Number of reflections | 23884 | 1197 |
| <I/σ(I)> | 24.5 | 2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 12.7 | 13.2 |
| CC(1/2) | 0.987 | 0.879 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 298.15 | 100 mM MES, pH 6.4, 100 mM Lithium acetate, 12% PEG3350 |
