8VDR
Cryogenic electron microscopy model of full-length talin without R12 and FABD
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
EM Data Collection
| Microscope | JEOL CRYO ARM 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Energy Filter | In-column Omega Filter |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.7 |
| Software | Topaz,cryoSPARC,PHENIX,SerialEM,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
