8VDL

HB3VAR03 CIDRa1.4 domain with C7 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97387
Spacegroup name	P 2 21 21
Unit cell lengths	55.653, 65.353, 250.341
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.300 - 2.680
R-factor	0.2192
Rwork	0.217
R-free	0.26590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.061
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.070	2.810
High resolution limit [Å]	2.680	2.680
Rmerge	0.143	1.531
Rmeas	0.151	1.621
Rpim	0.049	0.523
Total number of observations	250859	31447
Number of reflections	26449	3368
<I/σ(I)>	11.2	1.6
Completeness [%]	99.6
Redundancy	9.5	9.3
CC(1/2)	0.997	0.656

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.1M MES, pH 6.0, 0.2M Zn Acetate, 10% PEG 8K