8VD1

Crystal Structure of KAI2 from Oryza sativa with MPD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	298
Detector technology	PIXEL
Collection date	2022-05-24
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.999992
Spacegroup name	P 1 21 1
Unit cell lengths	37.711, 86.477, 44.617
Unit cell angles	90.00, 114.94, 90.00

Refinement procedure

Resolution	40.490 - 1.290
R-factor	0.15304
Rwork	0.152
R-free	0.16812
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.925
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.490	1.310
High resolution limit [Å]	1.290	1.290
Number of reflections	64764	2848
<I/σ(I)>	19.7
Completeness [%]	99.3
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1M Sodium HEPES, 0.1M MOPS (acid) pH 7.5, 0.018M Magnesium chloride hexahydrate, 0.018M Calcium chloride dihydrate, 9.4% v/v MPD, 9.4% v/v PEG 1000, 9.4% v/v PEG 3350