8VD1
Crystal Structure of KAI2 from Oryza sativa with MPD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 298 |
Detector technology | PIXEL |
Collection date | 2022-05-24 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.999992 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.711, 86.477, 44.617 |
Unit cell angles | 90.00, 114.94, 90.00 |
Refinement procedure
Resolution | 40.490 - 1.290 |
R-factor | 0.15304 |
Rwork | 0.152 |
R-free | 0.16812 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.925 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.490 | 1.310 |
High resolution limit [Å] | 1.290 | 1.290 |
Number of reflections | 64764 | 2848 |
<I/σ(I)> | 19.7 | |
Completeness [%] | 99.3 | |
Redundancy | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M Sodium HEPES, 0.1M MOPS (acid) pH 7.5, 0.018M Magnesium chloride hexahydrate, 0.018M Calcium chloride dihydrate, 9.4% v/v MPD, 9.4% v/v PEG 1000, 9.4% v/v PEG 3350 |