8VBQ

Structure of bovine anti-HIV Fab Bess7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-20
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97741
Spacegroup name	C 1 2 1
Unit cell lengths	83.002, 69.784, 87.521
Unit cell angles	90.00, 103.52, 90.00

Refinement procedure

Resolution	48.290 - 2.100
R-factor	0.2333
Rwork	0.232
R-free	0.26770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.290	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.152	1.070
Rmeas	0.179	1.280
Rpim	0.093	0.703
Number of reflections	28113	2607
<I/σ(I)>	8.7	1.2
Completeness [%]	98.8	92.4
Redundancy	3.5	3
CC(1/2)	0.992	0.376

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	0.095M sodium citrate, pH 5.6, 19% 2-propanol, 5% glycerol, 19% Peg4000