8VBP

Structure of bovine anti-HIV Fab Bess4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	66.607, 70.283, 111.459
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.940 - 2.800
R-factor	0.1984
Rwork	0.197
R-free	0.23170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.576
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.940	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.191	0.805
Rmeas	0.224	0.959
Rpim	0.115	0.510
Number of reflections	12710	1143
<I/σ(I)>	7	3
Completeness [%]	93.8	87.2
Redundancy	3.5	3.3
CC(1/2)	0.973	0.498

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	70% MPD, 0.1M Hepes, pH 7.5