8VBP
Structure of bovine anti-HIV Fab Bess4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.607, 70.283, 111.459 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.940 - 2.800 |
| R-factor | 0.1984 |
| Rwork | 0.197 |
| R-free | 0.23170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.576 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.940 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.191 | 0.805 |
| Rmeas | 0.224 | 0.959 |
| Rpim | 0.115 | 0.510 |
| Number of reflections | 12710 | 1143 |
| <I/σ(I)> | 7 | 3 |
| Completeness [%] | 93.8 | 87.2 |
| Redundancy | 3.5 | 3.3 |
| CC(1/2) | 0.973 | 0.498 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 70% MPD, 0.1M Hepes, pH 7.5 |
