8VBO

Structure of bovine anti-HIV Fab ElsE9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-22
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 2
Unit cell lengths	70.107, 150.891, 51.318
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.300 - 2.300
R-factor	0.2493
Rwork	0.247
R-free	0.28080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.530
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.300	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.092	0.778
Rmeas	0.108	0.909
Rpim	0.056	0.462
Number of reflections	24380	2325
<I/σ(I)>	9.3	2.5
Completeness [%]	97.8	95.3
Redundancy	3.3	3.4
CC(1/2)	0.995	0.611

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1M sodium cacodylate, pH 6.5, 40% MPD, 5% Peg8000