8VBO
Structure of bovine anti-HIV Fab ElsE9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-22 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 70.107, 150.891, 51.318 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.300 - 2.300 |
| R-factor | 0.2493 |
| Rwork | 0.247 |
| R-free | 0.28080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.530 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.300 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.092 | 0.778 |
| Rmeas | 0.108 | 0.909 |
| Rpim | 0.056 | 0.462 |
| Number of reflections | 24380 | 2325 |
| <I/σ(I)> | 9.3 | 2.5 |
| Completeness [%] | 97.8 | 95.3 |
| Redundancy | 3.3 | 3.4 |
| CC(1/2) | 0.995 | 0.611 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M sodium cacodylate, pH 6.5, 40% MPD, 5% Peg8000 |
