8VBM

Structure of bovine anti-HIV Fab ElsE7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	58.215, 70.612, 123.974
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.230 - 2.540
R-factor	0.2371
Rwork	0.235
R-free	0.28970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.506
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.230	2.630
High resolution limit [Å]	2.540	2.540
Rmerge	0.164	1.350
Rmeas	0.186	1.550
Rpim	0.086	0.742
Number of reflections	16971	1511
<I/σ(I)>	7.5	1.5
Completeness [%]	96.8	85.6
Redundancy	4.5	4.2
CC(1/2)	0.990	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	277	0.1M CHES, pH 9.5, 20% Peg 8000