8VBL

Structure of bovine anti-HIV Fab ElsE6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 2
Unit cell lengths	117.591, 69.983, 77.318
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.800 - 2.350
R-factor	0.1997
Rwork	0.198
R-free	0.24460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.540
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.800	2.430
High resolution limit [Å]	2.350	2.350
Rmerge	0.198	1.400
Rmeas	0.217	1.520
Rpim	0.086	0.594
Number of reflections	27206	2649
<I/σ(I)>	9.1
Completeness [%]	99.7	99.7
Redundancy	6.2	6.4
CC(1/2)	0.993	0.497

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	293	0.1M CHES, pH 9.5, 50% Peg200