8VBK

Structure of bovine anti-HIV Fab ElsE5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03317
Spacegroup name	P 21 21 21
Unit cell lengths	56.261, 70.018, 109.082
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.860 - 1.890
R-factor	0.2064
Rwork	0.204
R-free	0.24490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.633
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.860	1.960
High resolution limit [Å]	1.890	1.890
Rmerge	0.118	1.090
Rmeas	0.123	1.140
Rpim	0.035	0.323
Number of reflections	34653	3286
<I/σ(I)>	13.8	2.4
Completeness [%]	98.7	0.949
Redundancy	12.2	12.1
CC(1/2)	0.998	0.867

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1M sodium cacodylate, pH 5.5, 40% MPD, 5% Peg8000