8VAW

Magnesium catalyzed primer extension product with 14mer primer and mixed phosphorothioate activated G monomer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	99
Detector technology	PIXEL
Collection date	2023-04-25
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97648
Spacegroup name	P 3 2 1
Unit cell lengths	43.570, 43.570, 84.342
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.733 - 1.250
Rwork	0.188
R-free	0.19080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.022
RMSD bond angle	2.926
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.270
High resolution limit [Å]	1.250	1.250
Rmerge	0.044	0.424
Rmeas	0.042	0.499
Rpim	0.014	0.255
Number of reflections	27390	968
<I/σ(I)>	50.2	2.9
Completeness [%]	98.4	82
Redundancy	8.4	3.5
CC(1/2)	0.989	0.842

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	2.5 M Sodium chloride, 50 mM TRIS pH 7.5, 0.25 M Magnesium chloride.