8V9Y

X-ray crystal structure of nanobody JGFN4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	91.243, 26.858, 36.962
Unit cell angles	90.00, 106.99, 90.00

Refinement procedure

Resolution	43.630 - 1.760
R-factor	0.193
Rwork	0.191
R-free	0.23210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.599
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.630	1.810
High resolution limit [Å]	1.760	1.760
Rmerge	0.061	0.278
Rmeas	0.070	0.226
Rpim	0.035	0.359
Number of reflections	7992	400
<I/σ(I)>	10.4
Completeness [%]	91.4
Redundancy	3.6	2.2
CC(1/2)	0.998	0.901

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	RD22EH53, G4.1 20 w/v polyethylene glycol monomethyl ether 2000, 0.1 M Tris pH8.5, 0.2 M Trimethylamine N-oxide