8V9H
GES-5-NA-1-157 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.727, 80.381, 87.706 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.500 - 1.500 |
| R-factor | 0.1614 |
| Rwork | 0.160 |
| R-free | 0.19170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.087 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.500 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmeas | 0.094 | |
| Rpim | 0.031 | 0.416 |
| Number of reflections | 87364 | 4276 |
| <I/σ(I)> | 15.2 | 1.9 |
| Completeness [%] | 99.9 | |
| Redundancy | 9.5 | |
| CC(1/2) | 0.999 | 0.803 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 277 | Crystallized spontaneously from 20 mM HEPES. Protein concentration 15 mg/ml |
