8V99
GII.26 Leon 4509 norovirus protruding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2023-03-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95372 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.436, 71.165, 78.586 |
| Unit cell angles | 90.00, 109.92, 90.00 |
Refinement procedure
| Resolution | 40.660 - 1.470 |
| R-factor | 0.1502 |
| Rwork | 0.148 |
| R-free | 0.18710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.287 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.690 | 1.500 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.090 | 0.817 |
| Rmeas | 0.110 | 1.000 |
| Rpim | 0.062 | 0.569 |
| Total number of observations | 322323 | 14540 |
| Number of reflections | 111573 | 5215 |
| <I/σ(I)> | 6.1 | 0.9 |
| Completeness [%] | 98.8 | |
| Redundancy | 2.9 | 2.8 |
| CC(1/2) | 0.995 | 0.476 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.1M Ammonium acetate 0.1 M BIS-Tris (pH 5.5) 17% w/v PEG 10,000 |
