8V98
GII.24 Loreto 1972 norovirus protruding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2023-06-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.751, 80.821, 70.983 |
| Unit cell angles | 90.00, 113.62, 90.00 |
Refinement procedure
| Resolution | 45.840 - 1.740 |
| R-factor | 0.1618 |
| Rwork | 0.160 |
| R-free | 0.18930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.957 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.840 | 1.770 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.062 | 0.194 |
| Rmeas | 0.076 | 0.239 |
| Rpim | 0.043 | 0.136 |
| Total number of observations | 189441 | 10036 |
| Number of reflections | 63621 | 3390 |
| <I/σ(I)> | 12.1 | 4.3 |
| Completeness [%] | 98.8 | |
| Redundancy | 3 | 3 |
| CC(1/2) | 0.992 | 0.949 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | M Zinc sulfate heptahydrate M MES (pH 6.5) 25% v/v PEG 550 MME |
