8V8M
Switchgrass Chalcone Synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-11 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.050, 83.085, 106.450 |
| Unit cell angles | 79.43, 76.25, 77.21 |
Refinement procedure
| Resolution | 48.720 - 1.820 |
| R-factor | 0.2644 |
| Rwork | 0.264 |
| R-free | 0.28860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.782 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20_4459: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.720 | 1.885 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.145 | 0.605 |
| Number of reflections | 233811 | 21963 |
| <I/σ(I)> | 4.43 | 0.88 |
| Completeness [%] | 93.4 | 91.22 |
| Redundancy | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M Sodium Formate, 20% PEG 3350 |
