8V8K

Crystal Structure of Nanobody NbE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	80
Detector technology	PIXEL
Collection date	2015-08-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.033200
Spacegroup name	P 64 2 2
Unit cell lengths	76.559, 76.559, 298.596
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.370 - 2.850
R-factor	0.2906
Rwork	0.286
R-free	0.33130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.575
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.580	2.950
High resolution limit [Å]	2.850	2.850
Number of reflections	12993	1258
<I/σ(I)>	12.3	1.2
Completeness [%]	100.0
Redundancy	11.9
CC(1/2)	0.996	0.538

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	0.1 M Hepes pH 7.0 1.0M Succinic Acid pH 7.0 1% PEG 2000 MME