8V7J
Human DNA polymerase eta-DNA-araC-ended primer ternary complex:reaction with 20 mM Mg2+ for 600s
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2022-10-07 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.9786 |
Spacegroup name | P 61 |
Unit cell lengths | 98.722, 98.722, 82.093 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.920 - 1.660 |
R-factor | 0.2019 |
Rwork | 0.201 |
R-free | 0.22350 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.131 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 37.920 | 37.920 | 1.770 |
High resolution limit [Å] | 1.660 | 4.960 | 1.660 |
Rmerge | 0.060 | 0.029 | 0.727 |
Rmeas | 0.066 | 0.032 | 0.800 |
Number of reflections | 53145 | 3944 | 16736 |
<I/σ(I)> | 18.8 | ||
Completeness [%] | 99.7 | ||
Redundancy | 11.3 | ||
CC(1/2) | 0.999 | 0.999 | 0.795 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 2000MME, 0.1 M MES |