8V7H

Human DNA polymerase eta-DNA-araC-ended primer ternary complex:ground state at pH7.0 (K+ MES) with 1 Ca2+ ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.12723
Spacegroup name	P 61
Unit cell lengths	98.403, 98.403, 81.574
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.770 - 1.680
R-factor	0.2093
Rwork	0.208
R-free	0.23290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.139
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.770	37.770	1.790
High resolution limit [Å]	1.680	5.030	1.680
Rmerge	0.046	0.027	0.819
Rmeas	0.048	0.029	0.862
Number of reflections	50730	3757	16115
<I/σ(I)>	25.27
Completeness [%]	100.0
Redundancy	20.4
CC(1/2)	1.000	0.999	0.858

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 2000MME, 0.1 M MES