8V7G

Human DNA polymerase eta-DNA-gemC-ended primer-dAMPNPP ternary complex with Mg2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-25
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9765
Spacegroup name	P 61
Unit cell lengths	98.360, 98.360, 82.047
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.590 - 1.520
R-factor	0.1849
Rwork	0.184
R-free	0.19840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.081
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.590	42.590	1.610
High resolution limit [Å]	1.520	4.520	1.520
Rmerge	0.037	0.017	0.550
Rmeas	0.042	0.019	0.631
Number of reflections	69693	5190	21813
<I/σ(I)>	23.93
Completeness [%]	99.6
Redundancy	9.9
CC(1/2)	1.000	1.000	0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 2000MME, 0.1 M MES