8V7F

Human DNA polymerase eta-DNA-araC-ended primer-dAMPNPP ternary complex with Mn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2022-09-21
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.9786
Spacegroup name	P 61
Unit cell lengths	98.700, 98.700, 82.324
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.740 - 2.200
R-factor	0.2049
Rwork	0.203
R-free	0.25170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.788
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.740	42.740	2.330
High resolution limit [Å]	2.200	6.530	2.200
Rmerge	0.086	0.029	0.660
Rmeas	0.095	0.032	0.724
Number of reflections	23247	1710	7254
<I/σ(I)>	15.9
Completeness [%]	99.0
Redundancy	11.4
CC(1/2)	0.998	0.990	0.804

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 2000MME, 0.1 M MES