8V7E

Human DNA polymerase eta-DNA-araC-ended primer-dAMPNPP ternary complex with Mg2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2022-09-21
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.9786
Spacegroup name	P 61
Unit cell lengths	98.759, 98.759, 81.970
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.300 - 1.820
R-factor	0.1736
Rwork	0.172
R-free	0.20380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.082
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.300	42.300	1.930
High resolution limit [Å]	1.820	5.410	1.820
Rmerge	0.050	0.020	0.690
Rmeas	0.055	0.022	0.760
Number of reflections	40872	3038	12941
<I/σ(I)>	19.43
Completeness [%]	99.9
Redundancy	11.4
CC(1/2)	0.990	1.000	0.799

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 2000MME, 0.1 M MES