8V7D
Human DNA polymerase eta-DNA-dT primer rCTP insertion ternary complex at pH7.0 (K+ MES) with 1 Ca2+ ion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2021-04-15 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.9786 |
Spacegroup name | P 61 |
Unit cell lengths | 99.058, 99.058, 81.386 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.310 - 1.950 |
R-factor | 0.1846 |
Rwork | 0.182 |
R-free | 0.22990 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.160 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.310 | 42.310 | 1.860 |
High resolution limit [Å] | 1.750 | 5.220 | 1.750 |
Rmerge | 0.075 | 0.022 | 2.324 |
Rmeas | 0.083 | 0.025 | 2.848 |
Number of reflections | 33629 | 3393 | 14433 |
<I/σ(I)> | 12.92 | ||
Completeness [%] | 99.8 | ||
Redundancy | 11.3 | ||
CC(1/2) | 0.999 | 1.000 | 0.144 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 2000MME, 0.1 M MES |