8V69

Nanorana parkeri saxiphilin:GTX2 (co-crystal)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.11614
Spacegroup name	H 3
Unit cell lengths	229.740, 229.740, 67.868
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.420 - 1.950
R-factor	0.1873
Rwork	0.187
R-free	0.21170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.238
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.420	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.096
Number of reflections	97333	9717
<I/σ(I)>	9.91	0.81
Completeness [%]	100.0	100
Redundancy	10
CC(1/2)	0.999	0.410

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277.15	20-25% v/v PEG400, 4-5% w/v PGA-LM, 100-200 mM sodium acetate, pH 5.0, GTX2&3:NpSxph molar ratio 1.2:1