8V66
Nanorana parkeri saxiphilin:GTX5 (co-crystal)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.11614 |
| Spacegroup name | H 3 |
| Unit cell lengths | 229.156, 229.156, 67.505 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.310 - 1.900 |
| R-factor | 0.1903 |
| Rwork | 0.190 |
| R-free | 0.21810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.892 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.310 | 1.968 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.136 | |
| Number of reflections | 104167 | 10420 |
| <I/σ(I)> | 8.15 | 1.19 |
| Completeness [%] | 100.0 | 99.99 |
| Redundancy | 15.1 | |
| CC(1/2) | 0.998 | 0.580 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277.15 | 20-25% v/v PEG400, 4-5% w/v PGA-LM, 100-200 mM sodium acetate, pH 5.0, GTX5:NpSxph molar ratio 1.2:1 |
