8V5X
UIC-1 mutant - UIC-1-L6A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-27 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.619920 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 50.801, 16.792, 20.200 |
| Unit cell angles | 90.00, 103.99, 90.00 |
Refinement procedure
| Resolution | 17.550 - 1.120 |
| R-factor | 0.1718 |
| Rwork | 0.167 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.814 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.550 | 1.160 |
| High resolution limit [Å] | 1.120 | 1.120 |
| Rmerge | 0.119 | 0.752 |
| Rmeas | 0.130 | 0.830 |
| Number of reflections | 6420 | 593 |
| <I/σ(I)> | 8.69 | 2.55 |
| Completeness [%] | 98.1 | 94.59 |
| Redundancy | 6.3 | 5.7 |
| CC(1/2) | 0.996 | 0.734 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SLOW COOLING | 298 | water, acetonitrile |
