8V5W

UIC-1 mutant UIC-1-B5T

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.61992
Spacegroup name	C 1 2 1
Unit cell lengths	53.956, 10.164, 32.761
Unit cell angles	90.00, 99.18, 90.00

Refinement procedure

Resolution	22.390 - 1.070
R-factor	0.1605
Rwork	0.159
R-free	0.17830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.755
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.390	1.108
High resolution limit [Å]	1.070	1.070
Rmerge		0.385
Rmeas	0.310	0.440
Rpim	0.126	0.209
Number of reflections	7816	7816
<I/σ(I)>	5.23	3.45
Completeness [%]	95.4
Redundancy	5.9
CC(1/2)	0.925	0.812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SLOW COOLING		298	methanol