8V5J
The Native DNA 16-mer Sequence 5'-GCTGGATTAATCCAGC-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-07 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.751419 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 38.829, 38.829, 159.676 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.610 - 1.190 |
| R-factor | 0.1899 |
| Rwork | 0.188 |
| R-free | 0.21020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.340 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.610 | 1.233 |
| High resolution limit [Å] | 1.190 | 1.190 |
| Rmerge | 0.041 | 0.330 |
| Rmeas | 0.044 | 0.347 |
| Rpim | 0.015 | 0.105 |
| Number of reflections | 15183 | 1491 |
| <I/σ(I)> | 32.38 | 7.18 |
| Completeness [%] | 98.3 | 98.42 |
| Redundancy | 9.9 | 10.6 |
| CC(1/2) | 0.998 | 0.980 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 295 | 10mM HEPES, pH=8.6, 600mM CaCl2, 36% PEG200 mixed 1:1 with 1mM DNA duplex |
