8V5F

Nan Regulatory Protein Full-length from Streptococcus pneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953739
Spacegroup name	P 21 21 21
Unit cell lengths	53.241, 109.226, 202.385
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.910 - 2.550
R-factor	0.2641
Rwork	0.262
R-free	0.31280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8tx9
RMSD bond length	0.006
RMSD bond angle	0.821
Data reduction software	XDS (20161205)
Data scaling software	Aimless (8.0.013)
Phasing software	MOLREP (8.0.012)
Refinement software	REFMAC (8.0.013)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.910	2.641
High resolution limit [Å]	2.550	2.550
Rmerge	0.188
Number of reflections	39440	3859
<I/σ(I)>	9.16	0.77
Completeness [%]	97.6	98.01
Redundancy	10.1	10.7
CC(1/2)	0.998	0.610

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293.15	0.2 M lithium sulfate, 0.1 M Bis-Tris, pH 5.5, 25% w/v PEG3350