8V52
Crystal structure of 2A10 Fab bound to Human TGF-beta3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-14 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.987 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 133.940, 47.200, 200.830 |
Unit cell angles | 90.00, 101.68, 90.00 |
Refinement procedure
Resolution | 49.170 - 2.500 |
R-factor | 0.223 |
Rwork | 0.221 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.220 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.170 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 75332 | 3072 |
<I/σ(I)> | 11 | |
Completeness [%] | 97.2 | |
Redundancy | 5.6 | |
CC(1/2) | 0.996 | 0.514 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 25% PEG 1000, 0.1M HEPES pH 7.5 |