8V4W

Crystal structure of Campylobacter concisus Csep1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	293
Detector technology	PIXEL
Collection date	2018-08-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.07
Spacegroup name	P 64
Unit cell lengths	85.792, 85.792, 55.194
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 1.400
R-factor	0.12852
Rwork	0.126
R-free	0.16679
Structure solution method	AB INITIO PHASING
RMSD bond length	0.012
RMSD bond angle	1.769
Data scaling software	XDS
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.030
Number of reflections	43067	43067
<I/σ(I)>	15.1
Completeness [%]	94.3
Redundancy	1
CC(1/2)	0.990	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	25% w/v PEG 4000, 100 mM ammonium acetate, 80 mM sodium acetate trihydrate (pH 4.6)