8V4T

The Native DNA 16-mer sequence 5'-GCTGCGTTAACGCAGC-3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979338
Spacegroup name	H 3 2
Unit cell lengths	38.324, 38.324, 163.022
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.740 - 1.470
R-factor	0.1968
Rwork	0.195
R-free	0.21070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.503
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.740	1.523
High resolution limit [Å]	1.470	1.470
Rmerge	0.054	0.498
Rmeas	0.057	0.524
Rpim		0.160
Number of reflections	8251	802
<I/σ(I)>	23.58	4.52
Completeness [%]	99.6	100
Redundancy	10.7	10.6
CC(1/2)	0.999	0.936

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.6	295	10mM HEPES, pH=8.6, 600mM CaCl2, 38% PEG200 mixed 1:1 with 1mM DNA duplex