8V4I

Structure of bovine anti-HIV Fab ElsE1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	C 2 2 21
Unit cell lengths	69.789, 84.117, 174.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.890 - 1.810
R-factor	0.2226
Rwork	0.221
R-free	0.25710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.657
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.890	1.880
High resolution limit [Å]	1.810	1.810
Rmerge	0.155	2.500
Rmeas	0.162	2.640
Rpim	0.046	0.772
Number of reflections	45028	3292
<I/σ(I)>	10.5	0.9
Completeness [%]	95.6	70.1
Redundancy	10.2	10
CC(1/2)	0.996	0.410

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	40% ethylene glycol, 0.1M HEPES, pH 7.5, 5% PEG3000