8V35
Crystal structure of HpsN from Cupriavidus pinatubonensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-07-11 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.032, 87.767, 134.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.956 - 1.940 |
Rwork | 0.219 |
R-free | 0.26200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.988 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.930 | 1.980 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.036 | 1.073 |
Rpim | 0.016 | 0.476 |
Number of reflections | 68501 | 4501 |
<I/σ(I)> | 15.5 | 1.1 |
Completeness [%] | 99.9 | |
Redundancy | 6.8 | |
CC(1/2) | 1.000 | 0.789 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | HEPES, magnesium chloride, PEG 4000 |