8V33
Crystal structure of S. aureus TarL N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9770 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 69.634, 69.634, 66.590 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.700 - 1.700 |
| R-factor | 0.1825 |
| Rwork | 0.180 |
| R-free | 0.22060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.088 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.700 | 1.761 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 20949 | 2053 |
| <I/σ(I)> | 24.6 | 2.02 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 39.6 | 2.02 |
| CC(1/2) | 0.999 | 0.718 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.8 M succinic acid pH 7 |
