8V30
Smooth Muscle Gamma Actin (ACTG2) Filament Mutant R40C
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK III |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
Method | HELICAL |
Resolution (Å) | 2.54 |
Software | cryoSPARC,PHENIX,EPU,Coot |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |