8V2Y
Room temperature X-ray Crystal Structure of FMN-bound long-chain flavodoxin from Rhodopseudomonas palustris
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2021-10-18 |
Detector | DECTRIS EIGER R 4M |
Wavelength(s) | 1.5406 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.692, 58.878, 60.898 |
Unit cell angles | 90.00, 105.47, 90.00 |
Refinement procedure
Resolution | 28.270 - 2.860 |
R-factor | 0.2249 |
Rwork | 0.219 |
R-free | 0.27520 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.785 |
Data reduction software | CrysalisPro |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.270 | 3.080 |
High resolution limit [Å] | 2.860 | 2.860 |
Rmerge | 0.129 | |
Rmeas | 0.153 | |
Number of reflections | 7368 | 1366 |
<I/σ(I)> | 9.11 | |
Completeness [%] | 97.8 | |
Redundancy | 3.6 | |
CC(1/2) | 0.922 | 0.842 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 287 | 0.3-0.6M magnesium formate, 15-20% polyethylene glycol (PEG) 3350 |