8V2X
Crystal Structure of the reconstruction of the worst case of the ancestral triosephosphate isomerase of the last opisthokont common ancestor obtained by bayesian inference
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-05 |
Detector | DECTRIS PILATUS3 R 200K-A |
Wavelength(s) | 1.54178 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 46.205, 59.535, 114.789 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.500 - 1.430 |
R-factor | 0.1784 |
Rwork | 0.178 |
R-free | 0.17930 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.039 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.200 | 1.450 |
High resolution limit [Å] | 1.430 | 1.430 |
Number of reflections | 58029 | 2619 |
<I/σ(I)> | 23.9 | 2.7 |
Completeness [%] | 98.0 | 90.6 |
Redundancy | 5.4 | 3 |
CC(1/2) | 0.997 | 0.860 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20% (w/v) PEG 3350 , 0.2 M NaF |