8V1K
Crystal structure of outer membrane lipoprotein carrier protein (LolA) from Francisella tularensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 19-ID |
Synchrotron site | NSLS-II |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-08-06 |
Detector | DECTRIS EIGER2 XE 9M |
Wavelength(s) | 0.9795 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 83.809, 84.550, 102.469 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.530 - 1.800 |
R-factor | 0.2006 |
Rwork | 0.199 |
R-free | 0.23540 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.867 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.21rc1_5150: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.280 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.094 | 0.894 |
Rmeas | 0.102 | 0.970 |
Rpim | 0.039 | 0.374 |
Total number of observations | 235202 | 13173 |
Number of reflections | 34082 | 1980 |
<I/σ(I)> | 9.2 | 1.6 |
Completeness [%] | 100.0 | |
Redundancy | 6.9 | 6.7 |
CC(1/2) | 0.996 | 0.754 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | Morpheus Fusion H10: 12.5% v/v MPD; 12.5% PEG 1000; 25%w/v PEG 3350, 0.05M Tris (base), 0.05M BICINE, 0.3% w/v Sodium-L ascorbate, 0.3% w/v Choline Chloride, 0.3% v/v D-Panthenol, 0.3% w/v Pyridoxine hydrochloride, 0.3% w/v Thiamine hydrochloride, FrtuB.17730.a.VM3.PB00127 at 6.2 mg/mL. Plate 13568 well H10 drop2. Puck: PSL-1012, Cryo: direct |