8V1K

Crystal structure of outer membrane lipoprotein carrier protein (LolA) from Francisella tularensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 19-ID
Synchrotron site	NSLS-II
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-08-06
Detector	DECTRIS EIGER2 XE 9M
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
Unit cell lengths	83.809, 84.550, 102.469
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.530 - 1.800
R-factor	0.2006
Rwork	0.199
R-free	0.23540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.867
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21rc1_5150: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.280	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.094	0.894
Rmeas	0.102	0.970
Rpim	0.039	0.374
Total number of observations	235202	13173
Number of reflections	34082	1980
<I/σ(I)>	9.2	1.6
Completeness [%]	100.0
Redundancy	6.9	6.7
CC(1/2)	0.996	0.754

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	Morpheus Fusion H10: 12.5% v/v MPD; 12.5% PEG 1000; 25%w/v PEG 3350, 0.05M Tris (base), 0.05M BICINE, 0.3% w/v Sodium-L ascorbate, 0.3% w/v Choline Chloride, 0.3% v/v D-Panthenol, 0.3% w/v Pyridoxine hydrochloride, 0.3% w/v Thiamine hydrochloride, FrtuB.17730.a.VM3.PB00127 at 6.2 mg/mL. Plate 13568 well H10 drop2. Puck: PSL-1012, Cryo: direct