8V1I
Crystal structure of human mascRNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-07-29 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1 |
Spacegroup name | H 3 2 |
Unit cell lengths | 137.063, 137.063, 135.805 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 31.990 - 2.300 |
R-factor | 0.2154 |
Rwork | 0.215 |
R-free | 0.22940 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.079 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AutoSol |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.990 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 21925 | 2172 |
<I/σ(I)> | 19.33 | |
Completeness [%] | 99.8 | |
Redundancy | 34 | |
CC(1/2) | 0.994 | 0.811 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2 M calcium chloride dihydrate, 0.1 M sodium acetate trihydrate and 20% (v/v) 2-propanol |