8V11

Structure of a Saccharomyces cerevisiae Ipl1 peptide Bound to dwarf Ndc80 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 2.0.1
Synchrotron site	ALS
Beamline	2.0.1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2023-06-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0309
Spacegroup name	P 43 21 2
Unit cell lengths	114.757, 114.757, 423.841
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.530 - 3.950
R-factor	0.2696
Rwork	0.267
R-free	0.32660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.126
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.530	4.448
High resolution limit [Å]	3.950	3.950
Rmerge	0.556	3.899
Rmeas	0.573	4.006
Rpim	0.135	0.909
Number of reflections	10981	785
<I/σ(I)>	6.7
Completeness [%]	42.6	10.5
Redundancy	17.4	19
CC(1/2)	0.993	0.609

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291.15	15% polyethylene glycol 2000 monomethyl ether,500 mM sodium chloride,50 mM Tris pH 7.5