8V0D

Ubch5B-RING3 of MIB1 fusion structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-20
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	28.985, 65.119, 135.931
Unit cell angles	90.00, 93.03, 90.00

Refinement procedure

Resolution	37.160 - 2.400
R-factor	0.2142
Rwork	0.213
R-free	0.24340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.955
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.160	2.440
High resolution limit [Å]	2.400	2.400
Rpim	0.057	0.288
Number of reflections	18194	932
<I/σ(I)>	16.86	1.8
Completeness [%]	91.3
Redundancy	3
CC(1/2)	0.990	0.903

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	4% v/v TacsimateTM pH 6.0, 12% w/v Polyethylene glycol 3,350