8V09
Crystal Structure of the reconstruction of the ancestral triosephosphate isomerase of the last opisthokont common ancestor obtained by bayesian inference with PGH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-01 |
| Detector | DECTRIS PILATUS3 R 200K-A |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 46.647, 59.467, 114.374 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.950 - 1.610 |
| R-factor | 0.1656 |
| Rwork | 0.165 |
| R-free | 0.17340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.296 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.950 | 1.650 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Number of reflections | 41912 | 2004 |
| <I/σ(I)> | 10.6 | 2.1 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 5.2 | 3.3 |
| CC(1/2) | 0.942 | 0.648 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 291 | 4% v/v Tacsimate, 12% w/v PEG 3350 |
