8V06

Crystal structure of mouse PLD3 co-crystallized with 5'Pi-ssDNA for 9 days

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 1
Unit cell lengths	54.155, 54.191, 202.774
Unit cell angles	96.61, 89.16, 90.10

Refinement procedure

Resolution	49.920 - 2.730
R-factor	0.2572
Rwork	0.255
R-free	0.29540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.639
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	60.000	60.000	2.800
High resolution limit [Å]	2.730	7.460	2.750
Rmerge	0.121	0.056	0.424
Rmeas	0.146	0.068	0.546
Rpim	0.080	0.037	0.340
Number of reflections	57208	2956	2215
<I/σ(I)>	7.6
Completeness [%]	95.5	98.4	75.9
Redundancy	3	3.3	1.9
CC(1/2)	0.967	0.994	0.592

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	1.6 M ammonium sulfate, 0.5 mM magnesium chloride, 0.1 M citric acid, pH 4.0