8UZT
Mitochondrial single-stranded binding protein bound to DNA
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 135.201, 135.201, 78.081 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.810 - 1.900 |
| R-factor | 0.1855 |
| Rwork | 0.185 |
| R-free | 0.20720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.847 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.810 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 55728 | 55728 |
| <I/σ(I)> | 30 | |
| Completeness [%] | 97.0 | |
| Redundancy | 13.1 | |
| CC(1/2) | 0.997 | 0.813 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG3350 and 200 mM sodium nitrate |
