8UZ7
Crystal structure of a novel triose phosphate isomerase identified on the shrimp transcriptome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-25 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.181 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.564, 84.253, 73.739 |
| Unit cell angles | 90.00, 92.69, 90.00 |
Refinement procedure
| Resolution | 55.450 - 1.700 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.19090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.831 |
| Data reduction software | HKL-2000 |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.450 | 1.763 |
| High resolution limit [Å] | 1.700 | 1.702 |
| Rmerge | 0.080 | 0.821 |
| Rmeas | 0.090 | 0.936 |
| Rpim | 0.446 | |
| Number of reflections | 91724 | 9794 |
| <I/σ(I)> | 13.73 | 2.22 |
| Completeness [%] | 93.1 | |
| Redundancy | 6.1 | |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | Index kit (Hampton Research) No. 83 (0.2 M MgCl2-6H2O, 0.1 M Bis-Tris pH 6.5, 25% W/V PEG-3350, 20% PEG-400 as cryo-protectant) |
