8UY3
Fem1B with FNIP1 and Tom20 fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979460 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 183.715, 183.715, 183.715 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.930 - 3.200 |
| R-factor | 0.2404 |
| Rwork | 0.239 |
| R-free | 0.26440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.528 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.100 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 4.283 | |
| Rmeas | 0.403 | 4.513 |
| Rpim | 0.124 | 1.415 |
| Number of reflections | 31515 | 4939 |
| <I/σ(I)> | 8.8 | 0.8 |
| Completeness [%] | 91.7 | |
| Redundancy | 20.1 | |
| CC(1/2) | 0.995 | 0.383 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 10 mg/mL protein complex in 50 mM HEPES pH 7.5, 500 mM NaCl, 1 mM TCEP were mixed in a 2:1 ratio with the reservoir solution containing 30% PEG 3350, 0.2M Ammonium Citrate pH 7 |
