8UVZ
Bacillus subtilis DHFR bound to NADP+ and folate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 61 |
| Unit cell lengths | 88.450, 88.450, 37.265 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.220 - 0.930 |
| R-factor | 0.1109 |
| Rwork | 0.110 |
| R-free | 0.12330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.671 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | CNS |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.220 | 0.950 |
| High resolution limit [Å] | 0.930 | 0.930 |
| Rmeas | 0.065 | 0.657 |
| Number of reflections | 110282 | 5386 |
| <I/σ(I)> | 20.9 | 2.8 |
| Completeness [%] | 100.0 | 99.1 |
| Redundancy | 11.3 | 9.3 |
| CC(1/2) | 0.999 | 0.873 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 10% PEG4000, 100 mM sodium acetate pH=5.0 |
