8UVX
CosR DNA bound form I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-10-30 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.125, 81.303, 68.073 |
Unit cell angles | 90.00, 106.14, 90.00 |
Refinement procedure
Resolution | 45.000 - 2.900 |
R-factor | 0.2439 |
Rwork | 0.241 |
R-free | 0.26790 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.099 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.004 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 13804 | 782 |
<I/σ(I)> | 10.14 | |
Completeness [%] | 87.7 | |
Redundancy | 6.9 | |
CC(1/2) | 0.980 | 0.898 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.02 M Sodium formate, 0.02 M Ammonium acetate, 0.02 M Sodiumcitrate, 0.02 M Sodiium potassium tartrate, 0.02 M Sodium oxamate, 0.01 M MES ph6.5, 40% v/v PEG 500 MME, 20% w/v PEG 20000 |